CRISPR/Cas Plasmids (S.thermophilus)

Gateway cloning plasmid set Streptococcus thermophilus

pDe-St1-CAS9 (Nuclease) sequence information

• Cas9 expression vector (Cas9 driven by the constitutive Ubi4-2 promotor from parsley)
• Binary Gateway destination vector (contains ccdB gene!)
• Bacterial selection: Spectinomycin (100 mg/l)
• Plant selection: Kanamycin (30 mg/l)

pEn-St1-Chimera (sgRNA) sequence information

• sgRNA coding vector (sgRNA can easily be customized for any target site of interest; sgRNA is driven by the Arabidopsis U6-26 promoter)
• Gateway entry vector
• Bacterial selection: Ampicillin (100 mg/l)