Methods
NOTE: for some important methods that are used by many, we try to provide validated Standard Operation Protocols (SOPs) that have been tested and reviewed.
| Lab Basics | |
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Chemical analytics
Quantitative physiology
- How to estimate binding affinity using Michaelis-Menten modelling - pdf
- How to show receptor binding using a competitor assay - pdf
Molecular Methods - RNA work Standard Operation Protocols (SOPs)
- SOP: Extraction NucleoZOL - pdf (updated 18.09.2023). Note: this kit will be the new default
still in use, but in the long term outdated:
- SOP: Which kit for what? - pdf (updated 17.07.2018)
- SOP: Extraction Analytik Jena Kit - pdf (updated 30.08.2018)
- SOP: Extraction Sigma Kit for grapevine leaves and rice roots - pdf (update 30.08.2018)
- SOP: Extraction Sigma Kit for wood samples - pdf (updated 17.07.2018)
- SOP: Extraction Roboklon Kit for cells - pdf (updated 14.06.2021)
Molecular Methods - others
- SOP: column-free plasmid extraction - pdf (updated 18.09.2023)
- SOP: extraction of genomic DNA by CTAB - pdf (updated 20.01.2023)
- SOP: purification of PCR product - pdf (updated 18.09.2023)
- workshop RT-qPCR - pdf
- cDNA synthesis - pdf
- DNA extraction (CTAB) - pdf
- BP reaction - pdf
- LR reaction - pdf
Cellular and Physiological Phenotyping
- Superoxide in leaf discs - assay - quantification
- Hydrogen peroxide in leaf discs - quantification
- Mitochondrial shape - pdf - spread sheet
- Duration of cell cycle - pdf - spread sheet
- Astaxanthin content per cell - pdf
- Leaf Decoloration in Response to Stress - pdf
- Downy Mildew Growth in Leaves - pdf
- Ligand Binding by competition - pdf
- Surface Wax Structure - pdf - spread sheet
Microtubule Quantification
- Coverslip assay (nucleation, elongation, bundling) - pdf
- Integrity in vivo - pdf
- Orientation in vivo - pdf
Tubulin Methods
- Synthesis of carboxy-EPC for affinity chromatography of tyr versus detyr-tubulin - pdf